Probing the physiology of ASH neuron in Caenorhabditis elegans using electric current stimulation.
نویسندگان
چکیده
Electrical stimulation has been widely used to modulate and study the in vitro and in vivo functionality of the nervous system. Here, we characterized the effect of electrical stimulation on ASH neuron in Caenorhabditis elegans and employed it to probe the neuron's age dependent properties. We utilized an automated microfluidic-based platform and characterized the ASH neuronal activity in response to an electric current applied to the worm's body. The electrically induced ASH neuronal response was observed to be dependent on the magnitude, polarity, and spatial location of the electrical stimulus as well as on the age of the worm.
منابع مشابه
An Automated Microfluidic Platform for Electrical Stimulation of the Nervous System of C. Elegans
We demonstrate the use of an automated microfluidic platform for acquiring electrical stimulation-evoked neuronal calcium transients in the roundworm C. elegans. Our approach combines the use of a microfluidic chip for trapping single worms, microfabricated electrodes for applying electrical stimulation and genetically expressed calcium indicators for monitoring neuronal activity. We show that ...
متن کاملIn vivo imaging of C. elegans ASH neurons: cellular response and adaptation to chemical repellents.
ASH sensory neurons are required in Caenorhabditis elegans for a wide range of avoidance behaviors in response to chemical repellents, high osmotic solutions and nose touch. The ASH neurons are therefore hypothesized to be polymodal nociceptive neurons. To understand the nature of polymodal sensory response and adaptation at the cellular level, we expressed the calcium indicator protein cameleo...
متن کاملDecision making in C. elegans chemotaxis to alkaline pH
Monitoring of environmental and tissue pH is critical for animal survival. The nematode, Caenorhabditis elegans (C. elegans), is attracted to mildly alkaline pH, but avoids strongly alkaline pH. However, little is known about how the behavioral switching or decision making occurs. Genetic dissection and Ca(2+) imaging have previously demonstrated that ASEL and ASH are the major sensory neurons ...
متن کاملDEG/ENaC but Not TRP Channels Are the Major Mechanoelectrical Transduction Channels in a C. elegans Nociceptor
Many nociceptors detect mechanical cues, but the ion channels responsible for mechanotransduction in these sensory neurons remain obscure. Using in vivo recordings and genetic dissection, we identified the DEG/ENaC protein, DEG-1, as the major mechanotransduction channel in ASH, a polymodal nociceptor in Caenorhabditis elegans. But DEG-1 is not the only mechanotransduction channel in ASH: loss ...
متن کاملA dual mechanosensory and chemosensory neuron in Caenorhabditis elegans.
After light touch to its nose, the nematode Caenorhabditis elegans halts forward locomotion and initiates backing. Here we show that three classes of neurons (ASH, FLP, and OLQ) sense touch to the nose and hence are required for this avoidance response. ASH, FLP, and OLQ have sensory endings that contain axonemal cilia. Mutant animals that have defective ciliated sensory endings as well as lase...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied physics letters
دوره 99 5 شماره
صفحات -
تاریخ انتشار 2011